DNA purification is a step in the sample preparation workflow that removes enzymes, salts and other contaminants from lysed samples and PCR products before subsequent applications like cloning and sequencing. It also eliminates unwanted PCR-induced artefacts, such as primer dimers and nucleotides not integrated. DNA purification is a crucial step in molecular biology and requires careful planning to ensure high quality, reliable results.
There are many different methods to eliminating DNA. The conventional methods for DNA isolation comprise a variety of steps, such as leukocyte separation or red blood cell lysis to remove heme protein inhibitors of the PCR reaction. They also include deproteinization, RNAse treatment and ethanol and isopropanol precipitation, and then finally, DNA elimination. The majority of these procedures require specific equipment, such as an electrophoresis system as well as a biosafety cabinet because of the dangers of intercalating dyes in the gel electrophoresis.
Other methods of DNA purification use spin columns or 96 well filter plates to eliminate contaminants by adsorbing them to the surface of the plate or column. These methods can be time-consuming particularly if you are working with many samples Artificial gene synthesis or if the columns have to be manually refilled.
Dipsticks dramatically reduce the number of steps involved in processing samples to three. They bind nucleic Acid using the cellulose-based cellulose wax and release them once water is present. This technique is especially useful in low-resource environments, like remote field sites as well as teaching labs. Its simplicity and speed (30 seconds for each sample) makes it highly suitable for diagnostic molecular applications like diagnostics as well as genotype screening and heterozygosity testing.
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